FastQCFastQC Report
Sun 19 Nov 2017
SRR957824_500K_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR957824_500K_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500000
Sequences flagged as poor quality0
Sequence length150
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGGAGAGGGGCGGGGAGGGGAAGAGGGGAGAATTCGGGGGGGGCCGG7120.1424No Hit
ATCGGGAGAGGGGCGGGGGGGGGAAGAGGGGAGAATTCGGGGGGGGCCGG5390.10779999999999999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGAG30200.0137.89662
GAGGGGC30150.0135.316768
ATCGGGA37050.0131.814591
CGGGAGA32700.0131.352683
AGGGGCG37200.0130.94889
GAGAGGG34500.0125.554776
GGAGAGG35850.0121.629615
GGGAGAG40550.0121.005714
TTGGGAG4250.0116.7728962
ATTGGGA6000.0113.881791
GAGGGTC2900.0106.6906368
AGGGTCG3500.0102.791639
AAGGGGC8350.0100.8219768
TCGGGAA8750.092.064442
TGGGAGA6650.089.798443
GGAGAGC8600.087.850995
GAGAGCG9450.079.949056
ACGGGAG4200.077.062872
AGAGGGG64200.073.6353157
AGAGCGG8900.071.954157
Produced by FastQC (version 0.11.5)